Electrowetting-on-dielectric (EWOD) for biochemical applications: Particle manipulation for separation in droplet microfluidics. Gaurav Jitendra Shah

ISBN: 9781109124019

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NOOKstudy eTextbook

192 pages


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Electrowetting-on-dielectric (EWOD) for biochemical applications: Particle manipulation for separation in droplet microfluidics.  by  Gaurav Jitendra Shah

Electrowetting-on-dielectric (EWOD) for biochemical applications: Particle manipulation for separation in droplet microfluidics. by Gaurav Jitendra Shah
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The strong trend towards point-of-care health services and personalized medicine in recent times has drawn great research interest in microfluidics technologies for portable, powerful and low-cost diagnostics. Due to its simple design, low powerMoreThe strong trend towards point-of-care health services and personalized medicine in recent times has drawn great research interest in microfluidics technologies for portable, powerful and low-cost diagnostics.

Due to its simple design, low power consumption and reconfigurability, droplet microfluidics driven by non-mechanical actuation, such as that enabled by electrowetting-on-dielectric (EWOD), has many advantageous features for a lab-on-a-chip.-The objective of this research was to identify and address challenges towards applying EWOD for biochemical applications.

The major part of this dissertation is devoted to combining particle manipulation techniques with EWOD for effective separation of the target species, a critical step in many if not most biochemical assays.-Two powerful techniques have been used in this work for particle manipulation on EWOD.

Magnetic manipulation is a simple, specific, powerful and inexpensive technique which can be performed with just a simple permanent magnet. Although commonly used in clinical labs and even continuous microfluidics, utilizing superparamagnetic microparticles (magnetic beads) on a droplet microfluidics platform posed a few challenges due to the difference in the force scenario because of the fluidic interfaces present with droplets.

The key elements of this difference were identified, and techniques for effective utilization on EWOD in the presence of the differences were developed.-The second particle manipulation technique integrated with EWOD was the optoelectronic tweezers (OET), an exciting technology that can apply optically controlled dielectrophoretic force on specific particles. Optically controlled individual particle manipulation at a tiny fraction of optical power required by optical tweezers is the unique feature of this technology developed by Prof.

Ming Wus group at University of California, Berkeley. Integrating OET with EWOD opens up tremendous possibilities, particularly for isolated single-cell studies.-Besides active particle manipulation for the collection of target species, many biological assays require the removal of the non-target species (i.e., impurities) for high purity. In addition to serial dilution to lower the impurities concentration, we have also proposed a technique to achieve high purity that employs chemically and electrically stabilized slender fluidic structures that can be controlled with EWOD, to allow selective transport of target species while minimizing non-specific transport.



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